Stability and decolourization ability of Trametes villosa laccase in liquid ultrasonic fields
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چکیده
منابع مشابه
Stability and decolourization ability of Trametes villosa laccase in liquid ultrasonic fields.
We report in this study that the sonication of laccase from Trametes villosa and bovine serum albumin promotes the formation of protein aggregates with high molecular weight. The formation of aggregates leads to the deactivation of the enzyme, fact that was confirmed by the analysis of the enzyme stability (half-life time) upon ultrasound treatment. This inactivation was mainly caused by the ra...
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Trametes villosa laccase was used for direct azo dye degradation, and the reaction products that accumulated after 72 h of incubation were analyzed. Liquid chromatography-mass spectrometry (LC-MS) analysis showed the formation of phenolic compounds during the dye oxidation process as well as a large amount of polymerized products that retain azo group integrity. The amino-phenol reactions were ...
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Xenobiotic chemicals can be transformed or covalently bound to humic materials by oxidoreductive enzymes present in terrestrial systems. Chemicals that are not substrates for oxidoreductive enzymes may undergo transformation in the presence of certain reactive compounds, which are often referred to as mediators. In this study, cyprodinil, a broad-spectrum fungicide, did not show any transformat...
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Two laccases have been purified to apparent electrophoretic homogeneity from the extracellular medium of a 2,5-xylidine-induced culture of the white rot basidiomycete Trametes villosa (Polyporus pinsitus or Coriolus pinsitus). These proteins are dimeric, consisting of two subunits of 63 kDa as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and have typical blue laccase...
متن کاملUltrasound-intensified laccase production from Trametes versicolor.
An efficient intermittent ultrasonic treatment strategy was developed to improve laccase production from Trametes versicolor mycelia cultures. The optimized strategy consisted of exposing 2-day-old mycelia cultures to 5-min ultrasonic treatments for two times with a 12-h interval at the fixed ultrasonic power and frequency (120 W, 40 kHz). After 5 days of culture, this strategy produced the hig...
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ژورنال
عنوان ژورنال: Ultrasonics Sonochemistry
سال: 2007
ISSN: 1350-4177
DOI: 10.1016/j.ultsonch.2006.07.005